Hennache Fatima Zahra

• Biodiversity Journal, 13 (4): 0969-0980

  Brixi Gormat Nassima, Bekhiche Khouloud, Hadj Ali Nour El Houda, Azza Hena & Hennache Fatima Zahra
  Antioxidant and in vitro anti-inflammatory activities of the crude extracts of Bunium pachypodum P.W. Ball (Apiales Apiaceae) tubers from Alger
  https://doi.org/10.31396/Biodiv.Jour.2022.13.4.969.980

  ABSTRACT
  The objective of present study is to valorize different extracts (aqueous, ethanol and acetone) of the tubers of Bunium pachypodum P.W. Ball (Apiales Apiaceae) plant, by carrying out a phytochemical screening, and determine total phenolic and flavonoid contents, then evaluate the antioxidant activity by DPPH method (2, 2-diphenyl 1-1picrylhydrazyl) and FRAP (Ferric reducing antioxidant power), as well as the anti-inflammatory activity by stabilization method of human red blood cells and inhibition human albumin protein denaturation method. Phytochemical analysis of extracts showed presence for the presence of flavonoids, quinones, reducing sugars and saponins. Comparison between the extracts showed that aqueous extract exhibited the highest total polyphenols and flavonoids contents, followed by ethanol extract. The antioxidant activity evaluated through the DPPH assay showed that acetone extract was the more active, followed by aqueous extract, but lower active than the control (ascorbic acid). In contrast, it is the aqueous extract that has shown the most important reducing power. However, no significant difference was observed between the three extracts. At 1000 μg/ml, aqueous extract showed the most significant anti-hemolytic activity with 54%, and at the concentration of 500μg/ml, it showed 70.19% inhibition of albumin denaturation, statistically similar (P > 0.05) to that of the reference anti-inflammatory drug.

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