Boualem Harfi

• Biodiversity Journal, 13 (4): 0869-0875

  Amira Benahmed, Boualem Harfi, Imen Benchikhelhocine, Ibtissem Benbelkacem, Abla Daas & Khaoula Cherouana
  Tissue culture techniques for callus induction in Mentha rotundifolia (L.) Huds and M. pulegium L. (Lamiales Lamiaceae)
  https://doi.org/10.31396/Biodiv.Jour.2022.13.4.869.875

  ABSTRACT
  Explants of two species of Mentha rotundifolia (L.) Huds and Mentha pulegium L. were evaluated for in vitro callus induction at Biotechnology Research Center (C.R.Bt), Algeria, during 2018, using MS medium supplemented with different hormonal combinations of NAA (naphthaleneacetic acid) and KIN (Kinetine) mg/l. Objective of our study is to develop a well-adopted technique for callus induction in these species by using MS medium with different concentrations of growth hormones. Internodes and leafs were used as explant source. Best response toward callus formation (100%) were observed for M. pulegium on the combinations M11 (0.1 mg/l NAA +0.5 mg/l KIN), M13 (1 mg/l NAA +0.5 mg/l KIN), M14 (1.5 mg/l NAA+0.5 mg/l KIN), M17 (0.5 mg/l NAA +1 mg/l KIN) and M19 (1.5 mg/l NAA+1 mg/l KIN). For the M. rotundifolia, the highest rate (68.75%) of callus formation was obtained on M6 (0.1 mg/l NAA+0.1 mg/l KIN). The best average callus surface of 84.68 mm² was obtained in M19 (1.5 mg/l NAA, 1 mg/l KIN) for M. pulegium and highest average callus surface (49.07 mm²) was obtained in M7 (0.5 mg/l NAA, 0.1 mg/l KIN) for M. rotundifolia. Leafs explant induce important average callus surface than the internode explant for the both species of Mentha studied. The callus texture was friable and had two types of color: white and brown. The result demonstrated a well-adopted protocol for callus induction in these species of mentha from different explants source.

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