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Amira Benahmed

  • Biodiversity Journal, 13 (4): 0869-0875

    Amira Benahmed, Boualem Harfi, Imen Benchikhelhocine, Ibtissem Benbelkacem, Abla Daas & Khaoula Cherouana
    Tissue culture techniques for callus induction in Mentha rotundifolia (L.) Huds and M. pulegium L. (Lamiales Lamiaceae)
    https://doi.org/10.31396/Biodiv.Jour.2022.13.4.869.875

    ABSTRACT
    Explants of two species of Mentha rotundifolia (L.) Huds and Mentha pulegium L. were evaluated for in vitro callus induction at Biotechnology Research Center (C.R.Bt), Algeria, during 2018, using MS medium supplemented with different hormonal combinations of NAA (naphthaleneacetic acid) and KIN (Kinetine) mg/l. Objective of our study is to develop a well-adopted technique for callus induction in these species by using MS medium with different concentrations of growth hormones. Internodes and leafs were used as explant source. Best response toward callus formation (100%) were observed for M. pulegium on the combinations M11 (0.1 mg/l NAA +0.5 mg/l KIN), M13 (1 mg/l NAA +0.5 mg/l KIN), M14 (1.5 mg/l NAA+0.5 mg/l KIN), M17 (0.5 mg/l NAA +1 mg/l KIN) and M19 (1.5 mg/l NAA+1 mg/l KIN). For the M. rotundifolia, the highest rate (68.75%) of callus formation was obtained on M6 (0.1 mg/l NAA+0.1 mg/l KIN). The best average callus surface of 84.68 mm2 was obtained in M19 (1.5 mg/l NAA, 1 mg/l KIN) for M. pulegium and highest average callus surface (49.07 mm2) was obtained in M7 (0.5 mg/l NAA, 0.1 mg/l KIN) for M. rotundifolia. Leafs explant induce important average callus surface than the internode explant for the both species of Mentha studied. The callus texture was friable and had two types of color: white and brown. The result demonstrated a well-adopted protocol for callus induction in these species of mentha from different explants source.

  • Biodiversity Journal, 12 (1): 0265-0272

    Nadia Khater, Amira Benahmed, Nesrine Zereg & Khaoula Cherouana
    Callogenesis induction of Ilex aquifolium L. (Aquifoliales Aquifoliaceae)
    https://doi.org/10.31396/Biodiv.Jour.2021.12.1.265.272

    ABSTRACT
    English Holly (Ilex aquifolium L.) belongs to the family Aquifoliaceae and is one of the native and rare species in Algeria which has many benefits. The natural cultivation of this important ecological species presents many problems and takes a long time because of the various factors that increase the risk of extinction. In this research callogenesis was studied in order to induce better callus and to study the effect of Murashige and Skoog (MS), Woody Plant Medium (WPM) and various combinations and concentrations of growth regulators using young leaves as explants. Callus induction was successfully performed in WPM and MS culture media at high rates and with an earlier response on WPM medium. The maximum callus percentage (100%) was obtained on WPM medium supplemented with the combination of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) as well as on MS medium supplemented with BAP and 2.4-dichlorophenoxyacetic acid (2.4-D). The best mean callus surface area (151.77 mm2) was obtained in WPM medium with (1 mg.L-1of BAP+ 2 mg.L-1 NAA). The addition of (2 mg.L-1 BAP and 4 mg.L-1 NAA) to the MS medium also produced a high mean callus surface area of 82.95 mm2. The callus texture was compact and had three types of color: white, brown and greenish. The results of this study made it possible for the first time to develop an effective new alternative method for callus induction of I. aquifolium.