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Nadia Khater

  • Biodiversity Journal, 12 (1): 0265-0272

    Nadia Khater, Amira Benahmed, Nesrine Zereg & Khaoula Cherouana
    Callogenesis induction of Ilex aquifolium L. (Aquifoliales Aquifoliaceae)
    https://doi.org/10.31396/Biodiv.Jour.2021.12.1.265.272

    ABSTRACT
    English Holly (Ilex aquifolium L.) belongs to the family Aquifoliaceae and is one of the native and rare species in Algeria which has many benefits. The natural cultivation of this important ecological species presents many problems and takes a long time because of the various factors that increase the risk of extinction. In this research callogenesis was studied in order to induce better callus and to study the effect of Murashige and Skoog (MS), Woody Plant Medium (WPM) and various combinations and concentrations of growth regulators using young leaves as explants. Callus induction was successfully performed in WPM and MS culture media at high rates and with an earlier response on WPM medium. The maximum callus percentage (100%) was obtained on WPM medium supplemented with the combination of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) as well as on MS medium supplemented with BAP and 2.4-dichlorophenoxyacetic acid (2.4-D). The best mean callus surface area (151.77 mm2) was obtained in WPM medium with (1 mg.L-1of BAP+ 2 mg.L-1 NAA). The addition of (2 mg.L-1 BAP and 4 mg.L-1 NAA) to the MS medium also produced a high mean callus surface area of 82.95 mm2. The callus texture was compact and had three types of color: white, brown and greenish. The results of this study made it possible for the first time to develop an effective new alternative method for callus induction of I. aquifolium.