Andrea Pulvirenti

• Biodiversity Journal, 10 (4): 493-494 - MONOGRAPH

  Vita Giaccone, Gianluigi Maria Lo Dico, Licia Pantano, Gaetano Cammilleri, Valentina Cumbo, Antonio Vella, Francesco Giuseppe Galluzzo, Ilaria Rizzuto, Barbara Randisi, Giulio Bagnato, Andrea Pulvirenti & Andrea Macaluso
  First report on the presence of Alloxan in Bleached Flour by LC-MS/MS Method
  https://doi.org/10.31396/Biodiv.Jour.2019.10.4.493.494

  ABSTRACT
  Alloxan is a ketone with a low molecular weight and neutral functional groups. In this work the presence of Alloxan in bread, pastry and cake bleached flour was investigate in order to verify possible risk for consumers related to the use of chemicals for flour bleaching.

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• Biodiversity Journal, 10 (4): 491-492 - MONOGRAPH

  Giovanni Lo Cascio, Barbara Randisi, Nicola Cicero, Valentina Cumbo, Andrea Pulvirenti, Andrea Macaluso, Michele Chetta, Gaetano Cammilleri, Rosaria Collura, Stefania Graci, Maria Drussilla Buscemi, Antonio Vella & Vincenzo Ferrantelli
  Determination of Chlorpyriphos in Sicilian peaches by Gascromatography-MSMS method coupled with quechers sample preparation procedure preparation
  https://doi.org/10.31396/Biodiv.Jour.2019.10.4.491.492

  ABSTRACT
  Chlorpyrifos is an organophosphate pesticide used to kill a number of pests including insects and worms. It is considered moderately hazardous to humans by the World Health Organization and is harmful for these classes of animals. Gas chromatography tandem mass mass spectrometry (GC-MS) was used for the quantification and confirmation of Chlorpyrifos pesticide residues in peaches. The analysis carried out on peach samples confirmed that chlorpyrifos are widely used in Sicilian territory so that 34 percent of the samples were contaminated by the pesticide.

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• Biodiversity Journal, 10 (4): 481-484 - MONOGRAPH

  Gianluigi Maria Lo Dico, Antonello Cicero, Licia Pantano, Giuseppe Giangrosso, Ignazio Munna, Andrea Pulvirenti, Francesca Ornella Assiria, Vincenza Lo Verde, Andrea Macaluso, Gaetano Cammilleri, Valentina Cumbo, Rosaria Collura, Stefania Graci, Maria Drussilla Buscemi, Antonio Vella & Vincenzo Ferrantelli
  Evaluation of Histamine level in the Red Tuna (Thunnus thynnus) of the Mediterranean Sea in 2010–2015
  https://doi.org/10.31396/Biodiv.Jour.2019.10.4.481.484

  ABSTRACT
  Histamine is a biogenic amine present in fish species associated with a high amount of histidine, and it can cause the ‘scombroid poisoning’. The Commission Regulation (EC) No 2073/2005 govern the criteria to analyze histamine in fishery products and specifies that the high performance liquid chromatography (HPLC) is the reference method. In this work, 664 samples were presented in the 2010–2015 shine, of which 46 (6.9%) were positive. With Chart 1 it can be noted that the number of samples has increased substantially in recent years (2013–2015) due to intensified official controls. On the contrary, the number of positives has decreased progressively.

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• Biodiversity Journal, 10 (4): 475-478 - MONOGRAPH

  Gaetano Cammilleri, Stefania Graci, Maria Drussilla Buscemi, Rosaria Collura, Antonella Costa, Gianluigi Maria Lo Dico, Francesca Ornella Assiria, Michele Chetta, Innocenzo Ezio Giangrosso, Francesco Giuseppe Galluzzo, Valentina Cumbo, Antonio Vella, Andrea Pulvirenti & Vincenzo Ferrantelli
  New report of Anikasis larvae from Blunthead Puffer, Sphoeroides pachygaster caught off Strait of Sicily
  https://doi.org/10.31396/Biodiv.Jour.2019.10.4.475.478

  ABSTRACT
  Aim of the present paper was to report and identify by morphological and molecular methods the presence of anisakid L3 larvae found in 7 specimens of Sphoeroides pachygaster caught off Strait of Sicily from 2012 and 2015. Nematode larvae (n=9) were collected from three fish samples: the larvae were morphologically identified as belonging to the genus Anisakis Type I and, stored in 70% ethanol, were underwent molecular identification at species level by PCR- RFLP analysis of the rDNA (ITS-1, 5.8S gene, and ITS-2) region. Sequencing of ITS regions and comparison with sequences in GenBank were also performed. The parasites were molecular identified as belonging to A. pegreffii that is the predominant species in Mediterranean Sea. This is a new report of Anisakis sp. in S. pachygaster. The blunthead puffer Sphoeroides pachygaster is a fish species of Atlantic origin: in the last years there are indeed numerous reports of this alien fish species in the Mediterranean included Italian Seas. The presence of the species Anisakis pegreffii may support the hypothesis of complete adaptation of S. pachygaster in the Mediterranean Sea.

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